Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 58, Issue 49, Pages 17758-17763Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201909313
Keywords
coacervate; enzyme; proteinosome; response-retaliation behavior; synthetic protocell
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Funding
- ERC Advanced Grant Scheme [740235]
- Engineering and Physical Sciences Research Council, UK [EP/L002957/1]
- BBSRC (BrisSynBio, Bristol Centre for Synthetic Biology) [BB/L01386X/1]
- BBSRC [BB/L01386X/1] Funding Source: UKRI
- EPSRC [EP/L002957/1] Funding Source: UKRI
- European Research Council (ERC) [740235] Funding Source: European Research Council (ERC)
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Two different artificial predation strategies are spatially and temporally coupled to generate a simple tit-for-tat mechanism in a ternary protocell network capable of antagonistic enzyme-mediated interactions. The consortium initially consists of protease-sensitive glucose-oxidase-containing proteinosomes (1), non-interacting pH-sensitive polypeptide/mononucleotide coacervate droplets containing proteinaseK (2), and proteinosome-adhered pH-resistant polymer/polysaccharide coacervate droplets (3). On receiving a glucose signal, secretion of protons from 1 triggers the disassembly of 2 and the released protease is transferred to 3 to initiate a delayed contact-dependent killing of the proteinosomes and cessation of glucose oxidase activity. Our results provide a step towards complex mesoscale dynamics based on programmable response-retaliation behavior in artificial protocell consortia.
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