Journal
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
Volume 58, Issue 48, Pages 17399-17405Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/anie.201910772
Keywords
bioanalytical chemistry; biosensor; CRISPR Cas12a (cpf1); electrochemistry; trans-acting cleavage
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Funding
- National Institute of Health [NIBIB:1-P41EB021911, R01DK113185]
- Wallace R. Persons Research Fund from Case Alumni Association
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An accurate, rapid, and cost-effective biosensor for the quantification of disease biomarkers is vital for the development of early-diagnostic point-of-care systems. The recent discovery of the trans-cleavage property of CRISPR type V effectors makes CRISPR a potential high-accuracy bio-recognition tool. Herein, a CRISPR-Cas12a (cpf1) based electrochemical biosensor (E-CRISPR) is reported, which is more cost-effective and portable than optical-transduction-based biosensors. Through optimizing the in vitro trans-cleavage activity of Cas12a, E-CRIPSR was used to detect viral nucleic acids, including human papillomavirus 16 (HPV-16) and parvovirus B19 (PB-19), with a picomolar sensitivity. An aptamer-based E-CRISPR cascade was further designed for the detection of transforming growth factor beta 1 (TGF-beta 1) protein in clinical samples. As demonstrated, E-CRISPR could enable the development of portable, accurate, and cost-effective point-of-care diagnostic systems.
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