Journal
ANALYTICAL CHEMISTRY
Volume 91, Issue 23, Pages 14808-14811Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b03173
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Funding
- Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI16C1984]
- National Research Foundation (NRF) - Ministry of Science, ICT, and Future Planning [2016R1D1A1B03931270, 2017R1D1A1B03027897, 2019R1A2C2002390]
- National Research Foundation of Korea [2019R1A2C2002390, 2016R1D1A1B03931270, 2017R1D1A1B03027897] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Effective intermolecular interaction is required between probe and target molecules for successful detection of biomarkers. Here, we demonstrate that localization of probes on DNA nanostructures improves detection sensitivity and reaction rate. The structural flexibility of DNA nanostructures enabled frequent intramolecular interactions among the localized probes. The Smoluchowski coagulation method and the coarse-grained molecular dynamic software oxDNA were used for theoretical estimation of inter- and intramolecular behaviors of the DNA nanostructures as well as adequate experiments verifying the improvements in sensitivity with probe localization. Remarkably, the probe-localized DNA nanostructure had an increased sensitivity up to 274 times higher than that of the same probes without localization. We believe this achievement represents a wide applicability as a potential design strategy for robust, reliable, and sensitive biosensors.
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