Journal
ANALYTICAL CHEMISTRY
Volume 91, Issue 22, Pages 14773-14779Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.analchem.9b04282
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [21775080, 21505082]
- Open Projects Fund of Shandong Key Laboratory of Carbohydrate Chemistry and Glycobiology, Shandong University [2019CCG07]
- project of Shandong Province Higher Educational Science and Technology Program [J16LC10]
Ask authors/readers for more resources
Exosomes are membrane-enclosed phospholipid extracellular vesicles. In spite of their great promise as noninvasive biomarkers for cancer diagnosis, sensitive detection of exosomes is still challenging. Herein, the detection of exosomes was changed to the detection of DNA after recognition of exosomes with its aptamers. CD63 aptamer and EpCAM aptamer were used for the detection of MCF-7 cell-secreted exosome. The recognition process was amplified through the movements of a three-dimensional DNA walker. And then, Exonuclease III- assisted electrochemical ratiometric sensor was applied for further signal amplification. Under optimal conditions, the detection limit of 1.3 x 10(4) particles/mL was obtained with excellent selectivity. Furthermore, clinical application test for the detection of exosomes in human serum was also verified.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
