Journal
ANALYTICAL BIOCHEMISTRY
Volume 582, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ab.2019.113351
Keywords
Alkaline polyethylene glycol; Whole blood; Direct genotyping
Funding
- National Natural Science Foundation of China [81772289]
- Shaanxi Provincial Nano-Biomedical Detection Innovation Team Founds [2017KCT-25]
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In order to simplify biological sample preparation to meet the demand of rapid genotyping, we improve alkaline polyethylene glycol (APEG) based on Chomczynski's procedure for the PCR-based lateral flow genotyping system, which enables the rapid and efficient direct genotyping from whole blood without DNA purification. The improved APEG has a high tolerance for extreme storage conditions. Testing whole blood with an abnormal hematological index indicates that APEG efficiency would not be influenced by pathological factors. Compared with sequencing, the accuracy of this genotyping system was 100% on testing with 200 clinical samples.
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