Journal
AICHE JOURNAL
Volume 65, Issue 12, Pages -Publisher
WILEY
DOI: 10.1002/aic.16837
Keywords
fibroblasts; fibrosis; hydrogels; multidimensional controlled cell culture; synthetic extracellular matrices
Categories
Funding
- National Institute of General Medical Sciences [P20GM104316, P30GM110758]
- National Science Foundation Career Award [DMR-1253906]
- Pew Charitable Trusts [0026178]
Ask authors/readers for more resources
Many cell behaviors are significantly affected by cell culture geometry, though it remains unclear which geometry from two- to three-dimensional (2D-3D) culture is appropriate for probing a specific cell function and mimicking native microenvironments. Toward addressing this, we established a 2.5D culture geometry, enabling initial cell spreading while reducing polarization to bridge between 2D and 3D geometries, and examined the responses of wound healing cells, human pulmonary fibroblasts, within it. To achieve this, we used engineered biomimetic hydrogels formed by photopolymerization, creating robust layered hydrogels with spread fibroblasts at the interface. We found that fibroblast responses were similar between 2D and 2.5D culture and different from 3D culture, with some underlying differences in mechanotransduction. These studies established the 2.5D cell culture geometry in conjunction with biomimetic synthetic matrices as a useful tool for investigations of fibroblast activation with relevance to the study of other cell functions and types.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available