Journal
ACS NANO
Volume 13, Issue 11, Pages 12830-12839Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsnano.9b05038
Keywords
in vivo imaging; second near-infrared window; quantum dot; myeloid-derived suppressor cell; molecular imaging
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Funding
- National Natural Science Foundation of China [21974104, 81874131, 81672668]
- National Key Research and Development Program [2017YFSF090107]
- National Postdoctoral Science Foundation of China [2012T50663]
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Monitoring specific immune cells in vivo will provide significant information for improving the therapeutic effect of immunotherapy. Herein, the in vivo two-color fluorescence molecular imaging of an important immune cell, myeloid-derived suppressor cell (MDSC), was realized by using quantum dot (QD)-based nanoprobes with non-overlap emission in the second near-infrared window (NIR-II, 1000-1700 nm). NIR-IIa and NIR-IIb QDs were conjugated with two MDSC-specific antibodies, respectively, and targeted the in vivo MDSCs together. Due to the suppressed photon scattering and diminished autofluorescence in the NIR-II window, the distribution of MDSCs in different organs and tissues was clearly revealed in a non-invasive way by the colocalization of two-color fluorescence from nanoprobes. The high-resolution imaging further confirmed the exact distribution of MDSCs in tumor immune microenvironment (TIME). Our results demonstrated that NIR-II fluorescence nanoprobes with molecular targeting ability provided a powerful tool for monitoring the dynamic change of immune cell populations in TIME in vivo, thus guiding the choice of clinical medicine and evaluating the therapeutic effect.
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