Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 11, Issue 48, Pages 44933-44940Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b08662
Keywords
nanobowl array; high-throughput screening bacteria; single-cell identification; surface charges
Funding
- National Key R&D project from Minister of Science and Technology, China [2016YFA0202703]
- National Natural Science Foundation of China [61971049, 61875015, 31571006, 81601629, 21801019]
- Beijing Natural Science Foundation [2182091]
- Beijing Municipal Science & Technology Commission [Z181100004418004]
- Research and Development Program of BIGC [Ec201808]
- Science and Technology Planning Project of Guangdong Province [2018B030331001]
- National Youth Talent Support Program
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High-throughput screening and fast identification of single bacterial cells are crucial for clinical diagnosis, bioengineering, and fermentation engineering. Although single-cell technologies have been developed extensively in recent years, the single-cell technologies for bacteria still need further exploration. In this study, we demonstrate an identification and screening technology for single bacterial cells based on a large-scale nanobowl array, which is well-ordered and size-adjustable for use with different kinds of bacteria. When the culture medium with monodispersed bacteria was placed on the nanobowl array, it successfully enabled loading of single bacterium into a single nanobowl. Because of the limitative size and depth of the nanobowls, mixture of different bacteria species could be screened according to their sizes. In addition, with the help of a low electrical current, the bacteria can be further screened according to their intrinsic surface charges. If combined with micromanipulation technology, high-throughput single bacterial selection can be achieved in future.
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