Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 11, Issue 47, Pages 44566-44572Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b13737
Keywords
carbon dots; two-step hydrothermal; calcium detection; static fluorescence quenching; cellular imaging
Funding
- National Key Research and Development Program of China [2017YFF0108600]
- National Natural Science Foundation of China [21803075]
Ask authors/readers for more resources
It is well known that the calcium ion is essential for maintaining life activities in living organisms, and it is of great significance to detect the intracellular calcium concentration. For the detection of calcium ions, we developed a new type of fluorescent carbon dots (CDs), whose surface was modified by ethylenebis(oxyethylenenitrilo)tetraacetic acid (EGTA) through a secondary hydrothermal method. This is a simple and convenient chemical preparation method because all reactions are carried out in the same autoclave, and the final product is directly the EGTA-modified CDs. The CDs exhibit bright blue fluorescence, and as the calcium concentration increases, the fluorescence intensity drops sharply. The fluorescence quenching correlates with the concentration of calcium ions and has a good linearity in the range of 15-300 mu M with a detection limit of 0.38 mu M. The experimental results confirmed that the detection of calcium ions by CDs is a static fluorescence quenching process. Also, cytotoxicity test and cellular imaging experiments have shown that the CDs are nontoxic and biocompatible.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available