Journal
ACS APPLIED MATERIALS & INTERFACES
Volume 11, Issue 44, Pages 41091-41099Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsami.9b16025
Keywords
polydimethylsiloxane; protein engineering; anchor peptide; liquid chromatography peak I; LCI; RGD; bioactive surface coating; cell adhesion
Funding
- European Commission (EUSMI) [731019]
- EU
- federal state of North Rhine-Westphalia [EFRE 300088302]
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Polydimethylsiloxane (PDMS) is a synthetic material with excellent properties for biomedical applications because of its easy fabrication method, high flexibility, permeability to oxygen, transparency, and potential to produce high-resolution structures in the case of lithography. However, PDMS needs to be modified to support homogeneous cell attachments and spreading. Even though many physical and chemical methods, like plasma treatment or extracellular matrix coatings, have been developed over the last decades to increase cell surface interactions, these methods are still very time-consuming, often not efficient enough, complex, and can require several treatment steps. To overcome these issues, we present a novel, robust, and fast one-step PDMS coating method using engineered anchor peptides fused to the cell-adhesive peptide sequence (glycine-arginine-glycine-aspartate-serine, GRGDS). The anchor peptide attaches to the PDMS surface predominantly by by simply dipping PDMS in a solution containing the anchor peptide, presenting the GRGDS sequence on the surface available for cell adhesion. The binding performance and kinetics of the anchor peptide to PDMS are characterized, and the coatings are optimized for efficient cell attachment of fibroblasts and endothelial cells. Additionally, the applicability is proven using PDMS-based directional nanotopographic gradients, showing a lower threshold of 5 mu m wrinkles for fibroblast alignment.
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