Journal
JOURNAL OF APPLIED RESEARCH ON MEDICINAL AND AROMATIC PLANTS
Volume 14, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.jarmap.2019.100224
Keywords
Phaleria macrocarpa; Ultrasonic assisted extraction; Mangiferin; Antioxidant activity; UHPLC-PDA
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Funding
- Universiti Malaysia Pahang [PRGS 190349]
- Ministry of Higher Education Malaysia [FRGS/1/2016/TK02/UMP/02/4 [RDU 160124]]
- UMP Post-Doctoral Fellowship in Research
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This paper elucidates the correlation between the yield of mangiferin extraction from Phaleria macrocarpa fruits to the total phenolic content (TPC), total flavonoid content (TFC) and antioxidant activity (AA). Mangiferin extraction was performed using an ultrasonic assisted extraction (UAE) method. The effect of particle size, solvent to solid ratio, solvent type, ethanol concentration, sonication amplitude and extraction time were studied. The best UAE condition was found using a particle size of 125-250 mu m, solvent to solid ratio of 40 mL/g, 40% ethanol, sonication amplitude of 60% and extraction time of 5 min, which produced 28.6 mg mangiferin/g DW, 78.7 mg GA/g DW, 263.2 mg QE/g DW and 57.2% DPPH-RSA. The regression analysis showed a significant (p < 0.05) correlation between the mangiferin yield and either TPC, TFC or AA. The finding reported in this work provides a useful method to predict the mangiferin yield based on TPC, TFC or AA, without needing the actual external standard.
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