Journal
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
Volume 7, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2019.00201
Keywords
Chlamydomonas reinhardtii; hydrogen production; genomics; transcriptomics; proteomics; metabolomics
Funding
- National Natural Science Foundation of China [31600284]
- Shanghai Engineering Research Center of Plant Germplasm Resources [17DZ2252700]
- Shanghai Pujiang Program [18PJD008]
- Open Funding Project of the State Key Laboratory of Bioreactor Engineering [2018021]
- Open Funding Project of the State Key Laboratory of Marine Resource Utilization in South China Sea (Hainan University) [2019002]
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This article summarizes the current knowledge regarding omics approaches, which include genomics, transcriptomics, proteomics and metabolomics, in the context of bio-hydrogen production in Chlamydomonas reinhardtii. In this paper, critical genes (HydA1, Hyd A2, Sulp, Tla1, Sta7, PFL1) involved in H-2 metabolism were identified and analyzed for their function in H-2 accumulation. Furthermore, the advantages of gene microarrays and RNA-seq were compared, as well as their applications in transcriptomic analysis of H-2 production. Moreover, as a useful tool, proteomic analysis could identify different proteins that participate in H-2 metabolism. This review provides fundamental theory and an experimental basis for H-2 production, and further research effort is needed in this field.
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