Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1858, Issue 3, Pages 613-617Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2015.07.009
Keywords
Droplet interface bilayer; Electroporation; Optical single channel recording; Total internal reflection fluorescence microscopy
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Funding
- ERC (StG: CoSMiC)
- EPSRC through the Life Sciences Interface Doctoral Training Centre at the University of Oxford
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Using total internal reflection fluorescence microscopy of droplet interface bilayers containing the potassium sensitive fluorophore APG-4, we imaged the ionic flux through individual electropores. We are able to monitor up to 30 individual pores in parallel and show voltage dependent responses in fluorescence that corresponds to the measured ionic current. These experiments help quantify the scope and current limitations of optical single channel recordings of potassium flux. This article is part of a Special Issue entitled: Pore-Forming Toxins edited by Mauro Dalla Serra and Franco Gambale. (C) 2015 Elsevier B.V. All rights reserved.
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