Journal
PHARMACEUTICS
Volume 11, Issue 8, Pages -Publisher
MDPI
DOI: 10.3390/pharmaceutics11080387
Keywords
DNA particle (DNAp); netropsin; minor groove binder; calcium; rolling circle amplification (RCA)
Categories
Funding
- Bio & Medical Technology Development Program of the National Research Foundation of Korea (NRF) - Korean Government [NRF-2016M3A9C6917402]
- Creative Materials Discovery Program through the National Research Foundation of Korea (NRF) - Ministry of Science and ICT (MSIT) [NRF-2017M3D1A1039423]
- National Research Foundation of Korea (NRF) - Korea government (MSIP) [NRF-2017R1E1A1A01075027]
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Control of the release properties of drugs has been considered a key factor in the development of drug delivery systems (DDSs). However, drug delivery has limitations including cytotoxicity, low loading efficiency, and burst release. To overcome these challenges, nano or micro-particles have been suggested as carrier systems to deliver chemical drugs. Herein, nano-sized DNA particles (DNAp) were manufactured to deliver netropsin, which is known to bind to DNA minor grooves. The rationally designed particles with exposed rich minor grooves were prepared by DNAp synthesis via rolling circle amplification (RCA). DNAp could load large quantities of netropsin in its minor grooves. An analytical method was also developed for the quantification of netropsin binding to DNAp by UV-visible spectrometry. Moreover, controlled release of netropsin was achieved by forming a layer of Ca2+ on the DNAp (CaDNAp). As a proof of concept, the sustained release of netropsin by CaDNAp highlights the potential of the DNAp-based delivery approach.
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