Journal
EXPERIMENTAL NEUROBIOLOGY
Volume 28, Issue 4, Pages 451-457Publisher
KOREAN SOC BRAIN & NEURAL SCIENCE, KOREAN SOC NEURODEGENERATIVE DISEASE
DOI: 10.5607/en.2019.28.4.451
Keywords
Jaw closing gamma-motoneuron; Glutamatergic synapse; Vesicular glutamate transporter; Immunohistochemistry; Electron microscopy
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Funding
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [NRF-2017R1A5A2015391, NRF-2017R1A2B2003561]
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Currently, compared to jaw-closing (JC) alpha-motoneurons, the information on the distribution and morphology of glutamatergic synapses on the jaw-closing ( JC) gamma-motoneurons, which may help elucidate the mechanism of isometric contraction of the JC muscle, is very limited. This study investigated the distribution and ultrastructural features of vesicular glutamate transporter 1 (VGLUT1)- and VGLUT2-immunopositive (+) axon terminals (boutons) on JC gamma-motoneurons by retrograde tracing with horseradish peroxidase, electron microscopic immunocytochemistry, and quantitative analysis. About 35% of the boutons on identified JC gamma-motoneurons were VGLUT+, and of those, 99% were VGLUT2+. The fraction of VGLUT1+ boutons of all boutons and the percentage of membrane of JC gamma-motoneurons covered by these boutons were significantly lower than those for the JC alpha-motoneurons, revealed in our previous work. The bouton volume, mitochondrial volume, and active zone area of the VGLUT2+ boutons on the JC gamma-motoneurons were uniformly small. These findings suggest that the JC gamma-motoneurons, in contrast to the JC alpha-motoneurons, receive generally weak glutamatergic synaptic input almost exclusively from VGLUT2+ premotoneurons that form direct synapse with motoneurons.
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