Journal
JOURNAL OF CLINICAL MEDICINE
Volume 8, Issue 9, Pages -Publisher
MDPI
DOI: 10.3390/jcm8091440
Keywords
direct pulp capping; surface pre-reacted glass filler; mu CT; mu XRF; RNA sequence
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Funding
- JSPS KAKENHI [JP16K20453, 17K06848, 17K11704]
- SECOM Science and Technology Foundation (SECOM)
- Grants-in-Aid for Scientific Research [17K06848, 17K11704] Funding Source: KAKEN
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The induction of tissue mineralization and the mechanism by which surface pre-reacted glass-ionomer (S-PRG) cement influences pulpal healing remain unclear. We evaluated S-PRG cement-induced tertiary dentin formation in vivo, and its effect on the pulp cell healing process in vitro. Induced tertiary dentin formation was evaluated with micro-computed tomography (mu CT) and scanning electron microscopy (SEM). The distribution of elements from the S-PRG cement in pulpal tissue was confirmed by micro-X-ray fluorescence (mu XRF). The effects of S-PRG cement on cytotoxicity, proliferation, formation of mineralized nodules, and gene expression in human dental pulp stem cells (hDPSCs) were assessed in vitro. mu CT and SEM revealed that S-PRG induced tertiary dentin formation with similar characteristics to that induced by hydraulic calcium-silicate cement (ProRoot mineral trioxide aggregate (MTA)). mu XRF showed Sr and Si ion transfer into pulpal tissue from S-PRG cement. Notably, S-PRG cement and MTA showed similar biocompatibility. A co-culture of hDPSCs and S-PRG discs promoted mineralized nodule formation on surrounding cells. Additionally, S-PRG cement regulated the expression of genes related to osteo/dentinogenic differentiation. MTA and S-PRG regulated gene expression in hDPSCs, but the patterns of regulation differed. S-PRG cement upregulated CXCL-12 and TGF-beta 1 gene expression. These findings showed that S-PRG and MTA exhibit similar effects on dental pulp through different mechanisms.
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