Journal
NEUROPHOTONICS
Volume 6, Issue 3, Pages -Publisher
SPIE-SOC PHOTO-OPTICAL INSTRUMENTATION ENGINEERS
DOI: 10.1117/1.NPh.6.3.035011
Keywords
multichannel; fiber photometry; integrated probe; axonal terminals
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Funding
- National Natural Science Foundation of China [61890952, 81671106, 31700933]
- 973Program [2015CB759500]
- National Key Research and Development Program of China [2016YFA0201403]
- Science Fund for Creative Research Group of China [61721092]
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Fiber photometry has been increasingly popular in neuroscience research in freely behaving animals. In combination with genetically encoded calcium indicators, it allows for real-time monitoring of neural activity in neuronal somata, dendrites, and axonal terminals. We developed a multichannel fiber photometry device to map the activity of axonal terminals in a restricted, 100-mu m-wide brain region in freely moving mice. This device consists of four bundled multimode fibers, each with a 50-mu m core diameter and a scientific complementary metal-oxide semiconductor camera to simultaneously acquire fluorescence. We achieved a sampling rate of 100 frames/s and sufficient sensitivity to acquire data from axonal terminals. To avoid interference with neighboring channels, the recording depth of each channel was restricted to <250 mu m. Furthermore, the small-corediameter fibers did not restrict mouse locomotion. Using the Ca2+ indicator GCaMP5G, we validated the system by recording Ca2+, signals in axonal terminals from the medial entorhinal cortex layer II to the hippocampal dentate gyrus (DG) in freely moving mice. We detected spatially separated Ca2+ signals at four different sites in the DG. Therefore, our multichannel fiber photometry device provides a simple but powerful method to functionally map axonal terminals in spatially confined brain areas of freely moving animals.(C) The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License.
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