Journal
BIOCHEMISTRY-MOSCOW
Volume 81, Issue 10, Pages 1198-1204Publisher
MAIK NAUKA/INTERPERIODICA/SPRINGER
DOI: 10.1134/S0006297916100175
Keywords
translation initiation; 48S ribosomal initiation complex; initiation factors; eIF4F; eIF4A; eIF4B; toe-printing
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Funding
- Russian Foundation for Basic Research [15-04-015-25a, 16-34-00304 mol-a]
- program of Presidium of the Russian Academy of Sciences
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The formation of ribosomal 48S initiation complexes at the start AUG codon of uncapped mRNA leader sequences was studied using the methodology of primer extension inhibition (toe-printing). The experiments were performed in the system composed of purified individual components required for translation initiation. The formation of ribosomal 48S initiation complexes at the initiation codon was tested depending on the presence of the initiation factors eIF4F, eIF4A, and eIF4B. Several mRNAs containing short leader sequences lacking the extended secondary structure were studied. It was found that 48S ribosomal complexes at mRNAs with such leaders were not formed in the absence of eIF4F. In contrast, the removal of either eIF4A or eIF4B from the experimental system was found to be dispensable for the formation of the 48S complex.
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