Journal
TRANSLATIONAL CANCER RESEARCH
Volume 8, Issue 4, Pages 1466-1475Publisher
AME PUBL CO
DOI: 10.21037/tcr.2019.07.33
Keywords
Interleukin-10 (IL-10); hepatocellular carcinoma (HCC); hypomethylation
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Funding
- Natural Science Foundation of Jiangsu [BE2016656]
- Changzhou Sci AMP
- Tech Program, China [CJ20179029, CE20175037]
- Health Talents Project for Jiangsu, China [ZDRCC2016020]
- High-level Health Talents of Changzhou City [2016CZLJ009]
- Changzhou Municipal Commission of Health and Family Planning [ZD201816]
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Background: Epigenetic alterations of tumor-associated genes contribute to the pathogenesis of virtually all cancer types. We evaluated the methylation status of the interleukin-10 (IL-10) gene promoter and assessed its association with IL-10 mRNA expression and clinical prognosis in hepatocellular carcinoma (HCC) patients. Methods: Methylation-specific polymerase chain reaction (MSP) and real-time polymerase chain reaction (PCR) were used to define the methylation index (MI) of the IL-10 gene and quantify IL-10 mRNA expression in 120 HCC samples and paired non-tumor tissues. Results: Mean MI was 0.47 in HCC specimens and 0.59 in non-tumor controls, and was associated with metastasis classification and serum alpha-fetoprotein (AFP) levels. IL-10 mRNA levels [mean-Delta Delta Ct of 1.678 in HCC cases with hypomethylation (Delta MI <= 0) and -0.18 in HCC cases with hypermethylation (Delta MI >0)] also correlated with metastasis classification and serum AFP. An association was detected between IL-10 mRNA and its gene's MI in HCC. Also, an association was found between IL-10 hypomethylation, but not IL-10 mRNA expression and reduced postoperative HCC survival. Conclusions: These results indicate that IL-10 promoter hypomethylation is associated with increased IL-10 mRNA levels and indicative of poor survival in HCC.
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