Significant action ofTridax procumbensL. leaf extract on reducing the TNF-α and COX-2 gene expressions in induced inflammation site in Swiss albino mice

The leaves of traditionally used herbal plantTridax procumbensL. contain lots of phytochemicals having potency to reduce inflammation. In this study, the ethanol extract of the leaves ofTridax procumbensL. was analysed for the phytochemicals by GC-MS. The anti-inflammatory activity was then studied with the extract of 10, 50, and 100 mg/kg b.wt in carrageenan-induced mice model by measuring the inflammatory oedema and by analysing the histopathology. The mRNA expression levels of TNF-alpha and COX2 genes were studied in the inflammatory site to explore the molecular action by reverse transcription PCR and qPCR analyses. A significant (P <= 0.01) reduction in mice paw inflammation and a recovered histology were observed in treated groups when compared to control group in 24 h. The RT-PCR results showed a significant (P <= 0.01) decrease in the expression levels of TNF-alpha and COX2 in terms of band density in treated mice compared to control group. The qPCR RQ values also were decreased in treated groups with respect to increasing doses (RQ values of 18.985 +/- 0.230, 12.140 +/- 1.121, 6.718 +/- 0.807 for TNF-alpha and 15.583 +/- 1.043, 7.725 +/- 1.013, 5.075 +/- 0.615 for COX2, respectively for the three doses) in comparison with the control group (TNF-alpha 27.107 +/- 2.254, COX2 20.626 +/- 1.477).Tridax procumbensL. can be, thus, used for the development of a safe, natural, anti-inflammatory drug as it showed a strong inhibitory action on inflammation by acting at molecular level.