Journal
ACS BIOMATERIALS SCIENCE & ENGINEERING
Volume 5, Issue 10, Pages 5470-5480Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsbiomaterials.9b01085
Keywords
inclusion bodies; cell motility; high throughput; tissue engineering concentration gradients; surface patterning; protein nanoparticles
Categories
Funding
- DGI [MOTHER MAT2016-80826-R, Dynamo MAT2013-50036EXP]
- Networking Research Center on Bioengineering, Biomaterials, and Nanomedicine (CIBER-BBN)
- GenCat [2017-SGR-918, 2017-SGR-229]
- GenCat (CERCA Programme)
- COST Action Between Atom and Cell [CA15126]
- European Social Fund [NFFA-654360]
- EU [NFFA-654360]
- Consejo Superior de Investigaciones Cientificas (CSIC)
- Asociacion Espanola Contra el Cancer (AECC)
- INIA (DOC-INIA)
- ICREA ACADEMIA award
- People Programme (Marie Curie Actions) of the 7th Framework Programme of the European Union (FP7/2007-2013) under REA [600388]
- Agency for Business Competitiveness ACCIO through a Tecniospring fellowship [TECSPR15-1-0015]
- Spanish Ministry of Economy and Competitiveness, through the Severo Ochoa Programme for Centres of Excellence in RD [SEV-2015-0496]
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Eighty areas with different structural and compositional characteristics made of bacterial inclusion bodies formed by the fibroblast growth factor (FGF-IBs) were simultaneously patterned on a glass surface with an evaporation-assisted method that relies on the coffee-drop effect. The resulting surface patterned with these protein nanoparticles enabled to perform a high-throughput study of the motility of NIH-3T3 fibroblasts under different conditions including the gradient steepness, particle concentrations, and area widths of patterned FGF-IBs, using for the data analysis a methodology that includes heat maps. From this analysis, we observed that gradients of concentrations of surface-bound FGF-IBs stimulate the total cell movement but do not affect the total net distances traveled by cells. Moreover, cells tend to move toward an optimal intermediate FGF-IB concentration (i.e., cells seeded on areas with high IB concentrations moved toward areas with lower concentrations and vice versa, reaching the optimal concentration). Additionally, a higher motility was obtained when cells were deposited on narrow and highly concentrated areas with IBs. FGF-IBs can be therefore used to enhance migration, confirming that the decoration of surfaces with such IB-like protein nanoparticles is a promising platform for regenerative medicine and tissue engineering.
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