4.7 Article

GSTM3, but not IZUMO1, is a cryotolerance marker of boar sperm

Journal

Publisher

BMC
DOI: 10.1186/s40104-019-0370-5

Keywords

Boar; Cryopreservation; GSTM3; IZUMO1; ROS; Sperm

Funding

  1. Ministry of Science, Innovation and Universities, Spain [RYC-2014-15581, AGL2017-88329-R, FJCI-2017-31689]
  2. European Commission [H2020-MSCA-IF-79212]

Ask authors/readers for more resources

Background Cryopreservation is currently the most efficient method for long-term preservation of mammalian gametes and is extensively used in swine artificial insemination (AI) centres. However, it is well-known that cryopreservation procedures induce changes in the water phase in both intra and extracellular compartments, which alter the content and localisation of several proteins and ends up curtailing the structural integrity of functional sperm (i.e., cryoinjuries). Alterations and deficiencies of sperm-oocyte binding proteins during gamete recognition are one of the causes of reproductive failure both in vitro and in vivo. In this sense, characterisation of cryopreservation effects upon oocyte-binding proteins of sperm, such as IZUMO1 and GSTM3, is essential when assessing the impact of this technique in swine reproduction. Results Cryopreservation was found to induce changes in the localisation of IZUMO1 and GSTM3 in boar sperm. However, the relative content of both proteins was not altered after thawing. Furthermore, whereas IZUMO1 content was found not to be related to the cryotolerance of boar sperm, GSTM3 content was observed to be higher in poor (PFE) than in good (GFE) freezability ejaculates in both pre-frozen (1.00 INT center dot mm(2) +/- 0.14 INT center dot mm(2) vs. 0.72 INT center dot mm(2) +/- 0.15 INT center dot mm(2); P < 0.05) and post-thawed (0.96 INT center dot mm(2) +/- 0.20 INT center dot mm(2) vs. 70 INT center dot mm(2) +/- 0.19 INT center dot mm(2); P < 0.05) samples. Moreover, GSTM3 levels were found to be higher in those spermatozoa that exhibited low mitochondrial activity, high reactive oxygen species (ROS) production, and high membrane lipid disorder post-thaw (P < 0.05). Conclusions The difference in GSTM3 content between GFE and PFE, together with this protein having been found to be related to poor sperm quality post-thaw, suggests that it could be used as a cryotolerance marker of boar spermatozoa. Furthermore, both IZUMO1 and GSTM3 relocate during cryopreservation, which could contribute to the reduced fertilising capacity of frozen-thawed boar sperm.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.7
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available