Journal
CANCER IMMUNOLOGY RESEARCH
Volume 7, Issue 11, Pages 1748-1754Publisher
AMER ASSOC CANCER RESEARCH
DOI: 10.1158/2326-6066.CIR-19-0107
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Funding
- Lustgarten Foundation for Pancreatic Cancer Research
- Marcus Foundation
- Virginia and D.K. Ludwig Fund for Cancer Research
- Conrad R. Hilton Foundation
- Sol Goldman Center for Pancreatic Cancer Research
- Sol Goldman Sequencing Facility at Johns Hopkins
- Bloomberg Kimmel Institute at Johns Hopkins
- GI SPORE [CA 62924]
- ONCOLOGY CORE [CA 06973]
- MSTP [GM07309]
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Many immunotherapeutic approaches under development rely on T-cell recognition of cancer-derived peptides bound to human leukocyte antigen molecules on the cell surface. Direct experimental demonstration that such peptides are processed and bound is currently challenging. Here, we describe a method that meets this challenge. The method entailed an optimized immunoprecipitation protocol coupled with two-dimensional chromatography and mass spectrometry. The ability to detect and quantify minute amounts of predefined antigens should be useful both for basic research in tumor immunology and for the development of rationally designed cancer vaccines.
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