Journal
BIOCHEMICAL JOURNAL
Volume 473, Issue -, Pages 1343-1353Publisher
PORTLAND PRESS LTD
DOI: 10.1042/BCJ20160046
Keywords
dendritic cell-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN); glycan-binding proteins; glycan microarrays; glycan recognition; human milk glycans; lectins
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Funding
- National Institutes of Health [P41GM103694]
- Abbott Nutrition, Columbus, OH.
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Human milk glycans (HMGs) are prebiotics, pathogen receptor decoys and regulators of host physiology and immune responses. Mechanistically, human lectins (glycan-binding proteins, hGBP) expressed by dendritic cells (DCs) are of major interest, as these cells directly contact HMGs. To explore such interactions, we screened many C-type lectins and sialic acid-binding immunoglobulin-like lectins (Siglecs) expressed by DCs for glycan binding on microarrays presenting over 200 HMGs. Unexpectedly, DC-specific intercellular adhesion molecule-3-grabbing non-integrin (DC-SIGN) showed robust binding to many HMGs, whereas other C-type lectins failed to bind, and Siglec-5 and Siglec-9 showed weak binding to a few glycans. By contrast, most hGBP bound to multiple glycans on other microarrays lacking HMGs. An alpha-linked fucose residue was characteristic of HMGs bound by DC-SIGN. Binding of DC-SIGN to the simple HMGs 2'-fucosyl-lactose (2'-FL) and 3-fucosyl-lactose (3-FL) was confirmed by flow cytometry to beads conjugated with 2'-FL or 3-FL, as well as the ability of the free glycans to inhibit DCSIGN binding. 2'-FL had an IC50 of similar to 1 mMfor DC-SIGN, which is within the physiological concentration of 2'-FL in human milk. These results demonstrate that DC-SIGN among the many hGBP expressed by DCs binds to a-fucosylated HMGs, and suggest that such interactions may be important in influencing immune responses in the developing infant.
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