Journal
ELIFE
Volume 8, Issue -, Pages -Publisher
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.43582
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Funding
- European Research Council [310856]
- Schweizerischer Nationalfonds zur Forderung der Wissenschaftlichen Forschung [CRSII5_170925]
- Howard Hughes Medical Institute [55008733]
- European Molecular Biology Organization [ALTF 493-2015]
- Leibniz-Gemeinschaft [SAW-2017-FMP-1]
- Deutsche Forschungsgemeinschaft [SCHA 1274/4-1]
- Max-Planck-Gesellschaft
- Horizon 2020 Framework Programme PlantaSYST
- European Research Council (ERC) [310856] Funding Source: European Research Council (ERC)
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Many eukaryotic proteins regulating phosphate (Pi) homeostasis contain SPX domains that are receptors for inositol pyrophosphates (PP-InsP), suggesting that PP-InsPs may regulate Pi homeostasis. Here we report that deletion of two diphosphoinositol pentakisphosphate kinases VIH1/2 impairs plant growth and leads to constitutive Pi starvation responses. Deletion of phosphate starvation response transcription factors partially rescues vih1 vih2 mutant phenotypes, placing diphosphoinositol pentakisphosphate kinases in plant Pi signal transduction cascades. VIH1/ 2 are bifunctional enzymes able to generate and break-down PP-InsPs. Mutations in the kinase active site lead to increased Pi levels and constitutive Pi starvation responses. ATP levels change significantly in different Pi growth conditions. ATP-Mg2+ concentrations shift the relative kinase and phosphatase activities of diphosphoinositol pentakisphosphate kinases in vitro. Pi inhibits the phosphatase activity of the enzyme. Thus, VIH1 and VIH2 relay changes in cellular ATP and Pi concentrations to changes in PP-InsP levels, allowing plants to maintain sufficient Pi levels.
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