Journal
ELIFE
Volume 8, Issue -, Pages -Publisher
ELIFE SCIENCES PUBLICATIONS LTD
DOI: 10.7554/eLife.47642
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- Deutsche Forschungsgemeinschaft [SFB1009]
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Arrest of rapidly flowing neutrophils in venules relies on capturing through selectins and chemokine-induced integrin activation. Despite a long-established concept, we show here that gene inactivation of activating paired immunoglobulin-like receptor (PILR)-beta 1 nearly halved the efficiency of neutrophil arrest in venules of the mouse cremaster muscle. We found that this receptor binds to CD99, an interaction which relies on flow-induced shear forces and boosts chemokine-induced beta(2)-integrin-activation, leading to neutrophil attachment to endothelium. Upon arrest, binding of PILR-beta 1 to CD99 ceases, shifting the signaling balance towards inhibitory PILR-alpha. This enables integrin deactivation and supports cell migration. Thus, flow-driven shear forces guide sequential signaling of first activating PILR-beta 1 followed by inhibitory PILR-alpha to prompt neutrophil arrest and then transmigration. This doubles the efficiency of selectin-chemokine driven neutrophil arrest by PILR-beta 1 and then supports transition to migration by PILR-alpha.
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