Journal
CELL REPORTS
Volume 28, Issue 13, Pages 3497-+Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2019.08.051
Keywords
-
Categories
Funding
- NIH [R01GM116616, R01CA239161, F32 GM126646, 5T32CA009582]
- Breast Cancer Research Foundation
- Susan G. Komen fellowships [5T32CA009582, PDF14302198]
- Vanderbilt-Ingram Cancer Center
Ask authors/readers for more resources
Identifying proteins that function at replication forks is essential to understanding DNA replication, chromatin assembly, and replication-coupled DNA repair mechanisms. Combining quantitative mass spectrometry in multiple cell types with stringent statistical cutoffs, we generated a high-confidence catalog of 593 proteins that are enriched at replication forks and nascent chromatin. Loss-of-function genetic analyses indicate that 85% yield phenotypes that are consistent with activities in DNA and chromatin replication or already have described functions in these processes. We illustrate the value of this resource by identifying activities of the BET family proteins BRD2, BRD3, and BRD4 in controlling DNA replication. These proteins use their extra-terminal domains to bind and inhibit the ATAD5 complex and thereby control the amount of PCNA on chromatin.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available