Journal
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
Volume 475, Issue 4, Pages 329-334Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.bbrc.2016.05.095
Keywords
Lectin-like oxidized low-density lipoprotein receptor-1; GATA Binding Protein 4; Cardiac fibroblasts; Cell proliferation
Categories
Funding
- National Natural Science Foundation of China [81302892, 81400231, 81400234, 81503523]
- Guangdong Natural Science Foundation [2014A030313495]
- Science and Technology Planning Project of Guangdong Province [2014A020221059, 2014A020212330]
- Pearl River S&T Nova Program of Guangzhou [201506010071]
- Guangzhou Municipal university science and technology project [1201420465, 1201410344]
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Lectin-like oxidized low-density lipoprotein receptor-1 (LOX-1) and GATA Binding Protein 4 (GATA4) are important for the growth of cardiac fibroblasts (CFs). When deregulated, LOX-1 and GATA4 can cause cardiac remodeling. In the present study, we found novel evidence that GATA4 was required for the LOX-1 regulation of CF proliferation. The inhibition of LOX-1 by RNA interference LOX-1 lentivirus resulted in the loss of PI3K/Akt activation and GATA4 protein expression. The overexpression of LOX-1 by lentivirus rescued CF proliferation, PI3K/Akt activation, and GATA4 protein expression. Moreover, GATA4 over expression enhanced CF proliferation with LOX-1 inhibition. We also found that the inhibition of PI3K/Akt activation by LY294002, a PI3K inhibitor, reduced cell proliferation and protein level of GATA4. In summary, GATA4 may play an important role in the LOX-1 and PI3K/Akt regulation of CF proliferation. (C) 2016 Elsevier Inc. All rights reserved.
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