Journal
TRENDS IN MICROBIOLOGY
Volume 28, Issue 1, Pages 19-27Publisher
CELL PRESS
DOI: 10.1016/j.tim.2019.08.004
Keywords
-
Categories
Funding
- Science Foundation Ireland [13/IA/1875]
- EU Cooperation in Science and Technology action [CA17139]
- Science Foundation Ireland (SFI) [13/IA/1875] Funding Source: Science Foundation Ireland (SFI)
Ask authors/readers for more resources
In this opinion article we highlight links between the H-NS nucleoid-associated protein, variable DNA topology, the regulation of CRISPR-cas locus expression, activity, and the recruitment of novel genetic information by the CRISPR array. We prop the requirement that the invading mobile genetic element be negatively supercoiled limits effective CRISPR action to a window in the bacterial growth cycle when DNA topology is optimal, and that thin same window is used for the efficient integration of new spacer sequences at the CRISPR array H-NS silences CRISPR promoters, and we propose that antagonists of H-NS, such as the LeuC transcription factor, provide a basis for a stochastic genetic switch that acts at random in each cell in the bacterial population. In addition, we wish to propose a mechanism by which mobile genetic elements can suppress CRISPR-cas transcription using H-NS homologues. Although the in dividual components of this network are known, we propose a new model in which they are in tegrated and linked to the physiological state of the bacterium. The model provides a basis for cell-to-cell variation in the expression and performance of CRISPR systems in bacterial populations.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available