4.7 Editorial Material

Predicting CRISPR/Cas9-Induced Mutations for Precise Genome Editing

Journal

TRENDS IN BIOTECHNOLOGY
Volume 38, Issue 2, Pages 136-141

Publisher

CELL PRESS
DOI: 10.1016/j.tibtech.2019.08.002

Keywords

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Funding

  1. United States-India Educational Foundation (USIEF), New Delhi
  2. US Department of State
  3. National Science Foundation Plant Genome Research Program [1740874]
  4. USDA National Institute of Food and Agriculture and Hatch Appropriations [PEN04659, 1016432]
  5. Division Of Integrative Organismal Systems
  6. Direct For Biological Sciences [1740874] Funding Source: National Science Foundation

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SpCas9 creates blunt end cuts in the genome and generates random and unpredictable mutations through error-prone repair systems. However, a growing body of recent evidence points instead to Cas9-induced staggered end generation, nonrandomness of mutations, and the predictability of editing outcomes using machine learning models.

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