Journal
SENSORS AND ACTUATORS B-CHEMICAL
Volume 293, Issue -, Pages 281-288Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.snb.2019.05.020
Keywords
Cortisol; Nickel oxide; Immunosensor; Label-free; Saliva
Funding
- Department of Science and Technology (DST), Ministry of Science and Technology
- Department of Physics and Astrophysics, University of Delhi
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A non-invasive electrochemical immunosensor for detection of salivary cortisol has been proposed using RF sputtered NiO thin film as sensing platform. Cortisol, a steroid hormone, plays a vital part in administrating various physiological activities and also functions to be a stress biomarker. Thus, extremely sensitive and selective non-invasive mechanism for the detection of salivary cortisol has been focused in the present work. Covalently immobilized cortisol specific antibody on the NiO matrix has been used as the sensing platform. Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) studies have been implemented to calibrate the electrode using standard cortisol solutions. The label-free immunoelectrode has been successfully devised and used to achieve detection limit as low as 0.32 pg/mL with high sensitivity in a broad range of linearity from 1 pg/mL to 10 mu g/mL. It has been further assessed to detect cortisol in actual saliva samples and the results exhibit a great scope to foster the development of portable, integrated and efficient miniaturized non-invasive sensing devices for cortisol determination.
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