Journal
RESEARCH IN VETERINARY SCIENCE
Volume 125, Issue -, Pages 279-284Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2019.07.008
Keywords
Avian pathogenic Escherichia coli; pagP; Bacterial chemotaxis; Motility; ybjX
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Funding
- Natural Science Foundation of China [31802161, 31772707]
- Innovation Foundation for Postgraduates of Anhui Agricultural University [2019ysj-28]
- National Key R&D Program of China [2018YFD0500101]
- Introduction and stabilisation of Talent Research Project of Anhui Agricultural University [yj2017-31]
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Mutation of the PhoP/Q two-component system decreases the expression of ybjX and pagP encoding outer membrane proteins, and mutation of ybjX or pagP attenuates avian pathogenic Escherichia coli (APEC) pathogenicity. However, whether ybjX/pagP mutation (double-deletion mutant) has a synergistic effect on pathogenicity remains unknown. Herein, electrophoresis mobility shift assay (EMSA) experiments showed that the PhoP/Q system regulated ybjX and pagP transcription indirectly. The APEC Delta ybjX/pagP mutant strain, constructed using the Red recombination method, exhibited reduced invasion of chicken embryo fibroblast (DF-1) cells, but had no effect on virulence in a chicken model. Using RNA sequencing to identify differential mRNAs in APEC Delta ybjX Delta pagP and native strains, we revealed up-regulation of genes involved in the bacterial chemotaxis pathway. The ybjX/pagP mutant strain displayed significantly increased motility, suggesting that double deletion of ybjX and pagP enhances motility via the bacterial chemotaxis pathway.
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