Serum Apelin-36 alteration in late pregnancy and early lactation of dairy cows and its association with negative energy balance markers

The beneficial roles of Apelin on both energy metabolism and insulin sensitivity have been described in previous researches, but it has been little studied in dairy cows. The aim of the present study was to determine the serum Apelin-36 concentration in late pregnancy and early lactation in dairy cows and its association with negative energy balance markers. Thirty Holstein dairy cows (multiparous; n = 15 and primiparous; n = 15) with body condition score 3-3.75 at parturition were selected and blood samples were obtained for metabolic profile one month before and one month after parturition. Apelin-36, glucose, insulin, beta-hydroxybutyric acid (BHBA), non-esterified fatty acid (NEFA), cholesterol, triglyceride (TG) and high density lipoproteins (HDL) were measured using commercial kits. BCS and milk production were recorded during the study. There was no effect of parity on Apelin-36, cholesterol, TG, HDL, BHB and NEFA concentrations before lactation; while insulin and glucose levels were higher in primiparous cows than multiparous cows at this period. None of the factors showed any significant difference between multiparous and primiparous cows after lactation. Serum NEFA concentration were increased after parturition, while Apelin-36, insulin and glucose concentrations were decreased after parturition in primiparous and multiparous cows. Significant correlations were observed between serum Apelin and insulin (P = .041, r = 0.672), NEFA (P = .027, r = - 0.808) and glucose (P = .037, r = 0.757). In conclusion, our results showed that serum Apelin-36 concentration decreased after parturition in dairy cow. Alteration of Apelin-36 secretion after parturition may represent an endocrine adaptation in dairy cow during the lactating period.