4.4 Article

Expression of oestrogen receptor, androgen receptor and progesterone nuclear receptor in sheep uterus during the oestrous cycle

Journal

REPRODUCTION IN DOMESTIC ANIMALS
Volume 54, Issue 10, Pages 1305-1312

Publisher

WILEY
DOI: 10.1111/rda.13489

Keywords

androgen receptor; endometrium; oestrogen receptors; oestrous cycle; progesterone nuclear receptor; sheep uterus

Funding

  1. Gansu Province Key Research and Development Program -Agriculture [18YF1NA074]
  2. University Scientific Research Project of Education Department of Gansu Province [2018C-15]
  3. National Natural Science Foundation of China [31760754]
  4. Key Talent Project of Gansu Province [041045]

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Oestrogen, androgen and progesterone are involved in the regulation of uterine physiological functions, with the participation of the following proteins: oestrogen receptor (ER), androgen receptor (AR) and progesterone nuclear receptor (PGR). In this study, we used immunohistochemistry to detect the localization of ER alpha, ER beta, AR and PGR in sheep uterus. Additionally, we used real-time polymerase chain reaction (RT-qPCR) and Western blot technique to analyse their expression profiles at different stages of sheep oestrous cycle in the endometrium and myometrium. Immunohistochemical analysis showed that ER alpha, ER beta, AR and PGR were present in sheep uterus in oestrus, mainly in the uterine luminal epithelium, stroma, gland and myometrium. Real-time polymerase chain reaction results showed that in the endometrium, ER alpha expression level was highest in oestrus. ER beta and PGR, instead, were highly expressed in pro-oestrus. In the myometrium, ER alpha was highly expressed in both oestrus and pro-oestrus, and ER beta was highly expressed in oestrus and dioestrus. Progesterone nuclear receptor expression was highest in oestrus, followed by metoestrus. In the endometrium, both receptors ER alpha and ER beta were abundant in pro-oestrus, while the maximum AR protein content was found in oestrus. At this stage of the oestrous cycle, PGR protein concentration in the myometrium was significantly lower than those observed in other stages. These results suggest that these receptors are important for sheep reproductive function, as their expression at mRNA and protein levels exhibits particular time- and tissue-specific profiles along the oestrous cycle.

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