Journal
PLANT JOURNAL
Volume 101, Issue 1, Pages 112-121Publisher
WILEY
DOI: 10.1111/tpj.14522
Keywords
dual-color oligo-FISH; Oryza species; karyotype; chromosomal variation
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Funding
- National Natural Science Foundation of China [31571266, 31871232]
- Fund of Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- National Transgenic Major Project [2018ZX08020-003]
- Jiangsu Province Government Project [BK2018003]
- State Key Laboratory of Crop Genetics and Germplasm Enhancement [ZW201806]
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Fluorescence in situ hybridization using probes based on oligonucleotides (oligo-FISH) is a useful tool for chromosome identification and karyotype analysis. Here we developed two oligo-FISH probes that allow the identification of each of the 12 pairs of chromosomes in rice (Oryza sativa). These two probes comprised 25 717 (green) and 25 215 (red) oligos (45 nucleotides), respectively, and generated 26 distinct FISH signals that can be used as a barcode to uniquely label each of the 12 pairs of rice chromosomes. Standard karyotypes of rice were established using this system on both mitotic and meiotic chromosomes. Moreover, dual-color oligo-FISH was used to characterize diverse chromosomal abnormalities. Oligo-FISH analyses using these probes in various wild Oryza species revealed that chromosomes from the AA, BB or CC genomes generated specific and intense signals similar to those in rice, while chromosomes with the EE genome generated less specific signals and the FF genome gave no signal. Together, the oligo-FISH probes we established will be a powerful tool for studying chromosome variations and evolution in the genus Oryza.
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