Journal
PLANT AND SOIL
Volume 444, Issue 1-2, Pages 177-191Publisher
SPRINGER
DOI: 10.1007/s11104-019-04260-1
Keywords
Abscisic acid; BSMV-VIGS; iTRAQ; Nitrogen deficiency; Proteome; Triticum aestivum L
Categories
Funding
- Science and Technology Innovation Program for Increase in Yield and Efficiency of Food Crop [2016YFD0300105]
- National Key Technology Support Program [2015BAD26B01]
Ask authors/readers for more resources
Aims Crops often encounter a soil deficiency of nitrogen (N), the most important macronutrient for plants; however, the molecular mechanism of plant responses to N deficiency remains unclear. In this study, proteome-level changes that occur in bread wheat seedlings suffering from N deficiency were investigated to identify some N deficiency-responsive protein species in bread wheat. Methods We utilized isobaric tagging for relative and absolute quantification (iTRAQ) to measure changes in the proteome patterns of N-deficient wheat seedlings and validated the role of abscisic acid (ABA) using the barley stripe mosaic virus-induced gene-silencing (BSMV-VIGS) method. Results A total of 1515 N deficiency-responsive protein species were successfully identified in both root and leaf tissues of wheat seedlings suffering from 8-d N deficiency. Of these, abundance of wheat zeaxanthin epoxidase (TaZEP), a key ABA synthesis-related enzyme, was significantly upregulated, and the endogenous ABA contents also markedly increased. After TaZEP gene was further silenced using BSMV-VIGS method, BSMV-VIGS-TaZEP infected wheat seedlings showed enhanced sensitivity to N deficiency, suggesting silencing of TaZEP gene decreased the tolerance to N deficiency remarkably. Conclusion Our results identified some N deficiency-responsive protein species and revealed the role of ABA in wheat responses to N deficiency.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available