Antioxidant and Hypolipidemic Activities of Acid-Depolymerised Exopolysaccharides by Termitomyces albuminosus

The acid-depolymerised exopolysaccharides (ADES) of Termitomyces albuminosus were obtained, and the major fraction of ADES1 was isolated and purified by DEAE-52 cellulose anion-exchange column chromatography. Physicochemical characterizations showed that ADES1 was an alpha- and a beta-configuration with the molecular weight of 2.43 kDa, containing (1 -> 3, 4)-linked-Glcp, (1 -> 4)-linked-D-Glcp, (1 -> 3)-linked-D-Xylp, (1 -> 4)-linked-D-Manp, T-Glcp, (1 -> 6)-linked-D-Galp, and (1 -> 4)-linked-L-Arap. The in vivo assays showed that ADES1 could reduce lipid levels in the serum and liver, decrease serum enzyme activities, and improve antioxidant enzyme activities and p-AMPK alpha expressions in hyperlipidemic mice, which were also confirmed by histopathological observations. These data indicated that ADES1 might be considered as a novel substance to treat and prevent hyperlipidemia and as a hepatoprotective agent.