Journal
OPTICS LETTERS
Volume 44, Issue 17, Pages 4432-4435Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.44.004432
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Funding
- National Natural Science Foundation of China [61475103, 61775143]
- Department of Education of Guangdong Province [2017KZDXM073]
- China Postdoctoral Science Foundation [2019M653025]
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Here we demonstrate deep-brain 2-photon fluorescence microscopy in mouse in vivo, excited at the 1700 nm window. Through signal versus power measurement, we show that indocyanine green (ICG) is a promising 2-photon fluorescent dye excitable at the 1700 nm window. In order to excite ICG circulating in the vasculature in the deep brain, we employ a circular-polarization soliton self-frequency shift technique to generate energetic femtosecond pulses at 1617 nm. Combining the labeling and laser technologies, we achieve a record 2-photon fluorescence brain vasculature imaging depth of 2000 mu m in vivo. Both the effective attenuation length measurement and signalto-background ratio measurement indicate that we have reached the theoretical depth limit in 2-photon fluorescence microscopy. (C) 2019 Optical Society of America
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