Journal
OPTICS LETTERS
Volume 44, Issue 18, Pages 4451-4454Publisher
OPTICAL SOC AMER
DOI: 10.1364/OL.44.004451
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Funding
- Prostate Cancer Foundation
- U.S. Department of Defense [PC170176]
- National Science Foundation (NSF)
- Achievement Rewards for College Scientists Foundation
- NIH [F32 CA213615, K99 CA240681, R01 CA175391]
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Open-top light-sheet (OTLS) microscopy has been developed for rapid volumetric imaging of large pathology specimens. A challenge with OTLS microscopy is the transmission of oblique illumination and detection beams through a horizontal sample plate without introducing aberrations. Previous solutions prevented vertical sample movement, constrained the refractive index of the sample, and/or hindered multi-resolution imaging. Here we describe a solid immersion meniscus lens, a wavefront-matching element that suppresses aberrations when illumination and detection beam transition between air and various high-index immersion media, thereby enabling multi-resolution OTLS microscopy of specimens cleared with diverse protocols. (C) 2019 Optical Society of America
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