Journal
ONCOLOGY REPORTS
Volume 42, Issue 5, Pages 1793-1804Publisher
SPANDIDOS PUBL LTD
DOI: 10.3892/or.2019.7319
Keywords
melanoma; inflammation; microenvironment; cancer- associated fibroblasts; interleukin; proteomics
Categories
Funding
- Research, Development and Education Operational Programme [CZ.02.1.01/0.0/0.0/16_019/0000785]
- Research and Development for Innovations Operational Programme (RDIOP) - European Regional Development Fund [CZ.1.05/2.1.00/19.0400]
- state budget of the Czech Republic
- Grant Agency of the Czech Republic [16-05534S]
- Ministry of Health of the Czech Republic [16-29032A, 16-30954A]
- Charles University [PROGRESS Q 28]
- Ministry of Education, Youth and Sports of CR within the National Sustainability Programme I [LO1609]
- Ministry of Education, Youth and Sports of CR within the National Sustainability Programme II (Project BIOCEV-FAR) [LQ1604]
- project BIOCEV [CZ.1.05/1.1.00/02.0109]
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The steadily increasing incidence of malignant melanoma (MM) and its aggressive behaviour makes this tumour an attractive cancer research topic. The tumour microenvironment is being increasingly recognised as a key factor in cancer biology, with an impact on proliferation, invasion, angiogenesis and metastatic spread, as well as acquired therapy resistance. Multiple bioactive molecules playing cooperative roles promote the chronic inflammatory milieu in tumours, making inflammation a hallmark of cancer. This specific inflammatory setting is evident in the affected tissue. However, certain mediators can leak into the systemic circulation and affect the whole organism. The present study analysed the complex inflammatory response in the sera of patients with MM of various stages. Multiplexed proteomic analysis (Luminex Corporation) of 31 serum proteins was employed. These targets were observed in immunohistochemical profiles of primary tumours from the same patients. Furthermore, these proteins were analysed in MM cell lines and the principal cell population of the melanoma microenvironment, cancer-associated fibroblasts. Growth factors such as hepatocyte growth factor, granulocyte-colony stimulating factor and vascular endothelial growth factor, chemokines RANTES and interleukin (IL)-8, and cytokines IL-6, interferon-alpha and IL-1 receptor antagonist significantly differed in these patients compared with the healthy controls. Taken together, the results presented here depict the inflammatory landscape that is altered in melanoma patients, and highlight potentially relevant targets for therapy improvement.
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