Journal
NUCLEIC ACIDS RESEARCH
Volume 47, Issue 18, Pages 9592-9608Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkz691
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Funding
- Danish National Research Foundation, DNRF [99]
- Villumfonden [13169]
- Department of Plant and Environmental Sciences, University of Copenhagen
- Novo Nordisk Foundation SYNERGY Program
- Danish Research Councils [4181-00344]
- Danmarks Grundforskningsfond
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Physical interactions between members of the MYB and bHLH transcription factor (TF) families regulate many important biological processes in plants. Not all reported MYB-bHLH interactions can be explained by the known binding sites in the R3 repeat of the MYB DNA-binding domain. Noteworthy, most of the sequence diversity of MYB TFs lies in their non-MYB regions, which contain orphan small subgroup-defining motifs not yet linked to molecular functions. Here, we identified the motif mediating interaction between MYB TFs from subgroup 12 and their bHLH partners. Unlike other known MYB-bHLH interactions, the motif locates to the centre of the predicted disordered non-MYB region. We characterised the core motif, which enabled accurate prediction of previously unknown bHLH-interacting MYB TFs in Arabidopsis thaliana, and we confirmed its functional importance in planta. Our results indicate a correlation between the MYB-bHLH interaction affinity and the phenotypic output controlled by the TF complex. The identification of an interaction motif outside R3 indicates that MYB-bHLH interactions must have arisen multiple times, independently and suggests many more motifs of functional relevance to be harvested from subgroup-specific studies.
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