Journal
AVIAN PATHOLOGY
Volume 46, Issue 2, Pages 209-214Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/03079457.2016.1247432
Keywords
Salmonella Pullorum; Salmonella pathogenicity island 2; type III secretion system; gene expression; pseudogenes
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Funding
- Key Program of the National Natural Science Foundation of China [31230070]
- Colleges and Universities of Jiangsu Province Plans to Graduate Research and Innovation [CXZZ13_0917]
- Special Fund for Agroscientific Research in the Public Interest [201403054]
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Natural Science Foundation of Jiangsu Province of China [BK20151306]
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Salmonella pathogenicity island 2 (SPI2) can encode type III secretion system 2 (T3SS2) which plays an important role in systemic disease development through delivering different effector proteins into host cells. Here, the influence of Salmonella Pullorum pathogenicity island 2 on T3SS2 effector gene expression was studied using qRT-PCR in chicken macrophage HD11 cells. Our results showed that all the detected genes (including pseudogenes sifB, sspH2 and steC) can express in HD11 cells of S. Pullorum infection; deletion of SPI2 of S. Pullorum did not significantly affect the expression of genes cigR, gtgA, slrP, sopD, sseK1, steB and steC, but had a significant effect on the expression of genes pipB2, sifB, sopD2, sseJ, sseL, sspH2, steD, sifA, pipB and steA at different degrees. These results suggest that SPI2 can significantly affect the expression of some T3SS2 effector genes. Some effectors may have secretion pathways other than T3SS2 and pseudogenes may play roles in the process of S. Pullorum infection.
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