Journal
NEW PHYTOLOGIST
Volume 225, Issue 2, Pages 948-959Publisher
WILEY
DOI: 10.1111/nph.16169
Keywords
kinase proteins; resistance genes; Sr60; stem rust; Triticum monococcum; wheat
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Funding
- Howard Hughes Medical Institute
- Agriculture and Food Research Initiative Competitive Grant from USDA National Institute of Food and Agriculture (NIFA) [2017-67007-25939]
- USDA-ARS [5062-21220-023-00-D]
- USDA-ARS National Plant Disease Recovery System
- NRI Competitive Grant from the USDA National Institute of Food and Agriculture (NIFA) [2017-67007-25939]
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Wheat stem rust, caused by Puccinia graminis Pers. f. sp. tritici (Pgt), is a devastating fungal disease threatening global wheat production. The present paper reports the identification of stem rust resistance gene Sr60, a race-specific gene from diploid wheat Triticum monococcum L. that encodes a protein with two putative kinase domains. This gene, designated as WHEAT TANDEM KINASE 2 (WTK2), confers intermediate levels of resistance to Pgt. WTK2 was identified by map-based cloning and validated by transformation of a c.10-kb genomic sequence including WTK2 into susceptible common wheat variety Fielder (Triticum aestivum L.). Transformation of Fielder with WTK2 was sufficient to confer Pgt resistance. Sr60 transcripts were transiently upregulated 1 d post-inoculation with Pgt, but not in mock-inoculated plants. The upregulation of Sr60 was associated with stable upregulation of several pathogenesis-related genes. The Sr60-resistant haplotype found in T. monococcum was not found in polyploid wheat, suggesting an opportunity to introduce a novel resistance gene. Sr60 was successfully introgressed into hexaploid wheat, and we developed a diagnostic molecular marker to accelerate its deployment and pyramiding with other resistance genes. The cloned Sr60 also can be a useful component of transgenic cassettes including other resistance genes with complementary resistance profiles.
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