Journal
NATURE CHEMICAL BIOLOGY
Volume 15, Issue 9, Pages 882-+Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/s41589-019-0338-y
Keywords
-
Categories
Funding
- START grant from Japan Science and Technology Agency (JST) [JPMJCR1653]
- CREST grant from Japan Science and Technology Agency (JST) [JPMJCR1653]
- grants for Project for Cancer Research and Therapeutic Evolution Japan Agency for Medical Research and Development (AMED) [17cm0106415h0002]
- Japan Society for the Promotion of Science (JSPS)
- JSPS Research Fellowships for Young Scientists [15J05897]
- Grants-in-Aid for Scientific Research [15J05897] Funding Source: KAKEN
Ask authors/readers for more resources
The CRISPR-Cpf1 endonuclease has recently been demonstrated as a powerful tool to manipulate targeted gene sequences. Here, we performed an extensive screening of split Cpf1 fragments and identified a pair that, combined with inducible dimerization domains, enables chemical- and light-inducible genome editing in human cells. We also identified another split Cpf1 pair that is spontaneously activated. The newly generated amino and carboxyl termini of the spontaneously activated split Cpf1 can be repurposed as de novo fusion sites of artificial effector domains. Based on this finding, we generated an improved split dCpf1 activator, which has the potential to activate endogenous genes more efficiently than a previously established dCas9 activator. Finally, we showed that the split dCpf1 activator can efficiently activate target genes in mice. These results demonstrate that the present split Cpf1 provides an efficient and sophisticated genome manipulation in the fields of basic research and biotechnological applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available