Terminaliamide, a new ceramide and other phytoconstituents from the roots of Terminalia mantaly H. Perrier and their biological activities

Terminaliamide (1), a new ceramide was isolated from the roots of Terminalia mantaly H. Perrier (Combretaceae) along with 4 known compounds (2-5). The structures of the compounds were elucidated using 1D and 2D NMR spectroscopy analysis and mass spectrometry. Compound 1 exhibited moderated antibacterial activity towards Staphylococcus aureus with MIC value of 62.5 mu g/mL. The crude MeOH extract (TMr) highly reduced Plasmodium falciparum growth with an IC50 value of 10.11 mu g/mL, while hexane fraction (F1) highly reduced Trypanosoma brucei brucei growth with an IC50 value of 5.60 mu g/mL. All tested samples presented little or no in vitro cytotoxicity on HeLa cell line. The present work confirms that T. mantaly is medicinally important and may be used effectively as an antimicrobial, an antiplasmodial and an antitrypanosomial with promising therapeutic index.

Automated summary

A new ceramide, terminaliamide, was isolated from the roots of Terminalia mantaly along with 4 known compounds. Compound 1 showed moderate antibacterial activity against Staphylococcus aureus, while the crude MeOH extract highly reduced Plasmodium falciparum growth and the hexane fraction highly reduced Trypanosoma brucei brucei growth. All tested samples showed little or no cytotoxicity on HeLa cell line, indicating their potential as antimicrobial, antiplasmodial, and antitrypanosomal agents.