Anti-inflammatory and cytotoxic agents from Xylaria sp. SWUF09-62 fungus

The ongoing search for anti-cancer agents from microorganisms led to the isolation of four new compounds including 6-ethyl-8-hydroxy-4H-chromen-4-one (1), 6-ethyl-7,8-dihydroxy-4H-chromen-4-one (2), (3S)-3,4-dihydro-8-hydroxy-7-methoxy-3-methylisocoumarin (3) and (3S)-3,4-dihydro-5,7,8-trihydroxy-3-methylisocoumarin (4), together with eleven known compounds (5-15) from Xylaria sp. SWUF09-62 fungus. The chemical structures were deduced from IR, 1D and 2D NMR, and MS data. The absolute configurations of 3 and 4 were determined by ECD experiment. Compounds 2 and 4 indicated possible chemo-prevention and chemo-therapeutic properties, exhibited anti-inflammatory properties by reducing nitric oxide production in LPS-stimulated RAW264.7 cells (IC50 = 1.57 +/- 0.25 and 3.02 +/- 0.27 mu g/mL) and cytotoxicity against HT29 cells (IC50 = 16.46 +/- 0.48 and 97.78 +/- 7.14 mu g/mL).

Automated summary

The ongoing search for anti-cancer agents from microorganisms led to the isolation of four new compounds and eleven known compounds from Xylaria sp. SWUF09-62 fungus. Among them, compounds 2 and 4 showed potential chemo-prevention and chemo-therapeutic properties, as well as anti-inflammatory effects and cytotoxicity against certain cancer cells.