Journal
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
Volume 22, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.nano.2019.102093
Keywords
Gold nanorod; Chitosan; Multidrug resistant; Antibacterial; Antifungal; Inflammation; Cytotoxicity; Photothermal therapy
Funding
- Consejo Nacional de Ciencia y Tecnologia (CONACYT) [695756]
- Antibody Engineering and Proteomic facility, Immune and Infection Research Centre (IIRC), Jack Bell building, Vancouver, BC, Canada
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Plasmonic photothermal therapy (PPTT) has been used as an alternative to chemotherapy for the elimination of resistant microorganisms; however, its in situ evaluation has not been well studied. In the present study, we assessed the antimicrobial activity of a chitosan-based hydrogel embedded with gold nanorods (Ch/AuNRs) using a low power infrared diode laser. The antibacterial activity was measured in both Gram-positive and -negative strains, including clinical isolates of multidrug-resistant pathogens. The cytotoxic effect, cellular proliferation, and the expression of the pro-inflammatory (IL-6 and TNF-alpha) and anti-inflammatory (IL-10) cytokines were quantified in a murine model of macrophages. Results showed a potent antimicrobial activity of the Ch/AuNRs with MICs <= 4 mu g/mL, very low cytotoxicity with cell viability above 80%, and the macrophage proliferation was not affected for a period of 48 h. These results suggest that our Ch/AuNR-embedded hydrogel could be an option to locally control chronic nosocomial infections using PPTT. (C) 2019 Elsevier Inc. All rights reserved.
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