Journal
MOLECULAR GENETICS AND GENOMICS
Volume 294, Issue 6, Pages 1499-1509Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s00438-019-01592-6
Keywords
Ecl1 family gene; ecl1(+); fil1(+); Chronological lifespan; Leucine; Fission yeast
Funding
- Japan Society for the Promotion of Science KAKENHI [JP16K07662, JP17K19227, JP17H03792]
- Institute for Fermentation, Osaka
Ask authors/readers for more resources
Many studies show that lifespans of various model organisms can be extended by limiting the quantities of nutrients that are necessary for proliferation. In Schizosaccharomyces pombe, the Ecl1 family genes have been associated with lifespan control and are necessary for cell responses to nutrient depletion, but their functions and mechanisms of action remain uncharacterized. Herein, we show that leucine depletion extends the chronological lifespan (CLS) of leucine-auxotrophic cells. Furthermore, depletion of leucine extended CLS and caused cell miniaturization and cell cycle arrest at the G1 phase, and all of these processes depended on Ecl1 family genes. Although depletion of leucine raises the expression of ecl1(+) by about 100-fold in leucine-auxotrophic cells, these conditions did not affect ecl1(+) expression in leucine-auxotrophic fil1 mutants that were isolated in deletion set screens using 79 mutants disrupting a transcription factor. Fil1 is a GATA-type zinc finger transcription factor that reportedly binds directly to the upstream regions of ecl1(+) and ecl2(+). Accordingly, we suggest that Ecl1 family genes are induced in response to environmental stresses, such as oxidative stress and heat stress, or by nutritional depletion of nitrogen or sulfur sources or the amino acid leucine. We also propose that these genes play important roles in the maintenance of cell survival until conditions that favor proliferation are restored.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available