Journal
LWT-FOOD SCIENCE AND TECHNOLOGY
Volume 111, Issue -, Pages 252-259Publisher
ELSEVIER
DOI: 10.1016/j.lwt.2019.05.006
Keywords
Young apple polyphenols; alpha-Amylase inhibition; Inhibition kinetics; Fluorescence quenching; Differential scanning calorimetry
Categories
Funding
- China Agricultural Research System [CARS-28]
- Ministry of Agriculture of the People's Republic of China
Ask authors/readers for more resources
In this study, compositional profile, antioxidant activities and a-amylase inhibition of young apple polyphenols (YAP) from five cultivars (Short-branch Fuji, Pink Lady, Jinshiji, Ruiyang and Qinyang) were investigated. The possible inhibition mechanism of YAP on porcine pancreatic alpha-amylase (PPA) was studied through kinetic analysis, fluorescence quenching (FQ) and differential scanning calorimetry (DSC). The results showed that YAP exhibited strong antioxidant activities, and showed a significant inhibitory effect on PPA through a competitive inhibition mode. Jinshiji YAP exhibited the highest inhibitory capacity with the IC50 value of 0.023 mg/mL. Besides, combination of DSC and FQ results demonstrated that the potential unfolding of PPA structure was induced by binding with YAP.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
