Journal
JOURNAL OF VIROLOGICAL METHODS
Volume 270, Issue -, Pages 150-152Publisher
ELSEVIER
DOI: 10.1016/j.jviromet.2019.05.009
Keywords
Citrus leaf blotch virus; RT-LAMP; Detection; Kiwi fruit
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Citrus leaf blotch virus (CLBV) is a type member of the genus Citrivirus belonging to Betaflexiviridae. In this study, a reverse transcription loop-mediated isothermal amplification (RT-LAMP) method was developed to detect CLBV; this technology has been widely used in the detection of various plant pathogenic microorganisms and exogenous genes. The sensitivity of the RT-LAMP method was increased 100-fold compared to that of the conventional RT-PCR. In addition, this method was fast, simple and specific; it could provide better technical support for field diagnosis, customs quarantine and the control measures of CLBV. To our knowledge, this is the first report detecting CLBV using RT-LAMP.
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